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. 2016 Aug 13;7(38):61199–61214. doi: 10.18632/oncotarget.11284

Figure 6. Effect of miR-26a, miR-34a and miR-455-3p inhibitor on cell progression in MHCC97L cells.

Figure 6

(A) The expression of miR-26a, miR-34a and miR-455-3p was studied by qRT–PCR in MHCC97L cells transfected with the inhibitor (*P < 0.05). (B) MHCC97L cells were transfected with miR-26a, miR-34a and miR-455-3p respectively. Expression of miRNA was confirmed by qRT–PCR. B, Growth curves of miRNA inhibitor-transfected cells were compared to NC inhibitor cells with the CCK-8 assay (*P < 0.05). (C) Morphology foci were observed at the microscope and photographed. (D) Ki67 expression was detected by immunofluorescence staining in MHCC97L cells treated with miRNA inhibitor transfection. Red fluorescence: Ki67; DAPI staining for nuclear DNA. (E) Knockdown of miR-26a, miR-34a and miR-455-3p significantly increased the migration and invasion of MHCC97L cells. The data were mean ± S.D. of three separate transfections (*P < 0.05).