Figure 3. Representative MS/MS spectra of peptides of hyperphosphorylated proteins in H358-S cells.

(A) p21 activated kinase 6 (B) TAO kinase 3. (C) AKT mediates PAK6 phosphorylation in lung cancer cells exposed to cigarette smoke: H358-P and H358-S cells were treated with PF-3758309 and LY294002 respectively. Western blot was performed using phospho AKT, total AKT, phospho PAK6 and total PAK6 antibodies. β-actin was used as a loading control.