Figure 4. NRF2 protein (A) and apoptosis induction (B, C, D) in the cells treated with sulforaphane (SFN), IP3R blockers (block), or combination of both, SFN and blocker (comb).
To show the effect of the IP3R blockers, experiment with a less specific 2-aminoethoxydiphenyl borate (2-APB; black columns) or experiment with the more specific Xestospongin C (Xest; empty columns) was performed on A2780 cells A, B. Increase in the amount of apoptotic cells due to SFN treatment (20μM) was observed also in MDA-MB-231 cells (C; gray columns), Bowes cells (C; black columns) and SKOV3 cells (D, black columns). When all these cells were treated in parallel with SFN and Xest (comb), no increase compared to controls was observed B, C, D. Each column is displayed as mean ± S.E.M and represents an average of at least three independent cultivations, each performed in triplicates. Statistical significance ** compared to corresponding control represents p <0.01 and *** p <0.001. Statistical significance + compared to SFN treated group represents p <0.05, ++ p <0.01 and +++ p <0.001.