Figure 9. IP3R1 immunofluorescence of control (A, D) and SFN-treated (B, E) A2780 cells.

IP₃R1 was localized in the endoplasmic reticulum in control cells and partially translocates to the nuclei in SFN-treated cells (B; white arrows). In order to show that IP₃R1 are really in the nuclei, we performed z-stacks from control D. and SFN-treated E. cells. Orthogonal views D, E, F. give a middle plane of z-stacks crossing the nuclei and also xz and yz sections through the entire thickness of cells along green and red lines, clearly showing presence of the IP₃R1 signal (E) and also signal for ER marker - calnexin F. within nuclei in SFN treated cells. Signal was not observed in control, non-treated cells (D). As a negative control (C), cells were treated only with the secondary antibody. Nuclei stained by DAPI are shown in blue.