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. 2016 Aug 18;7(38):61426–61437. doi: 10.18632/oncotarget.11366

Figure 1. Upregulation of ICAM2 mRNA and protein by p53 family.

Figure 1

(A) Real-time RT-PCR analysis showed ICAM2 mRNA upregulation following overexpression of the p53 family genes into human cancer cell lines. The cells were infected with adenoviruses expressing FLAG-tagged p53 family genes at an MOI of 25 (Saos-2 and H1299) or 100 (SAS, Ca9-22 and KOSC3), and the cells were harvested 24 h after infection. Relative gene expression levels were quantified using the ΔΔCt method and the results were normalized to the expression of the housekeeping gene GAPDH. The data are shown as the mean ± standard errors of three independent experiments and were normalized to their respective controls as 1. (B) Upregulation of ICAM2 protein by p53 family members. Cells were infected with the indicated adenoviruses as described above and immunoblot analysis of FLAG, ICAM2, p21, and β-actin was performed.