Figure 5. Recombinant human ICAM2 (rhICAM2) alters cancer cell migration, invasion, and adhesion.

(A) Wound healing assay after addition of rhICAM2 (62.5 ng/mL) to SAS and Ca9-22 cells. Phase contrast images were taken at 0 and 8 h after wounding. Representative images showing decreased cell migration to the wounded area after addition of rhICAM2. (B) Cell invasion was measured in a Matrigel invasion assay after addition of rhICAM2 to SAS and Ca9-22 cells. The experiments were repeated three times with similar results. Representative images showing decreased cell invasion after addition of rhICAM2 (left). Quantification of invasion as a percentage of the control is shown (right). *p < 0.05; relative to untreated cells. (C) Cell adhesion was measured after addition of rhICAM2. Approximately 1 × 10³ cells with or without rhICAM2 were plated in triplicates on 96-well plates. After 60 min incubation, cell adhesion was measured as described in the Figure 4D legend. *p < 0.05; relative to untreated cells.