Skip to main content
. 2016 Aug 18;7(38):61426–61437. doi: 10.18632/oncotarget.11366

Figure 6. Regulation of the MEK-ERK pathway by ICAM2.

Figure 6

(A) ICAM2 siRNA enhanced ERK1/2 phosphorylation in cancer cells. After stable transfection of ICAM2 siRNA plasmid (3 populations, si-ICAM2-1, 2, and 3) or empty plasmid (si-cont) in HSC4 oral cancer cells, cell lysates (20 μg of protein) were subjected to immunoblot with antibodies against p-ERK1/2, ERK1/2, and β-actin. (B) The invasion-enhancing effect of ICAM2 siRNA was inhibited by the addition of MEK inhibitor. Cells were suspended in medium containing 0, 5, or 10 μM U-0126 (0.5 mL/chamber) and then subjected to invasion assays. The experiments were repeated at least three times independently. Quantification of invasion as a percentage of the control is shown (lower panels).