Figure 4. Impact of R(+)-methanandamide (MA) and Δ⁹-tetrahydrocannabinol (THC) on basal and IL-1β-induced TF expression.

Time dependencies of TF protein expression following incubation of cells with MA (A) or THC (B) in the presence or absence of IL-1β. Effect of AM-251 (CB₁ antagonist), AM-630 (CB₂ antagonist) and capsazepine (Capsa, TRPV1 antagonist) on the MA-mediated increase of IL-1β-induced (C) or basal TF protein levels (D). Impact of MA and THC on IL-1β-induced TF activity (E) and on IL-1β-mediated decrease of aPTT (F). Cells were incubated with MA at 10 μM or THC at 3 μM for the indicated times (A, B) or for 8 h (C–F) in the presence or absence of IL-1β (10 ng/ml). Receptor antagonists (C, D) were added to cultures 1 h prior to MA. Percent control represents comparison with vehicle-treated cells (100%) in the absence of test substance. Values are means + SEM of n = 3 (A, B, C, E) or n = 6 (D) per group. In the box plot (F, n = 17–18 per group), boxes extend from the 25th percentile to the 75th percentile, with a horizontal line inside the box at the median. Whiskers indicate minimum and maximum values, respectively. **P < 0.01, ***P < 0.001 vs. corresponding vehicle control; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. IL-1β, ^§§P < 0.01 vs. IL-1β + MA, ANOVA plus post Bonferroni test.