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. 2016 Aug 18;7(38):61438–61457. doi: 10.18632/oncotarget.11367

Figure 6. Role of MAPKs in IL-1β-induced TF expression and its mo.

Figure 6

dulation by WIN 55,212-2. (A) Effect of SB203580 (inhibitor of p38 MAPK; 10 μM), PD98059 (inhibitor of p42/44 MAPK activation; 10 μM) and SP600125 (inhibitor of JNK; 10 μM) on basal and IL-1β-induced TF protein levels. Cells were pretreated with the respective inhibitor for 1 h. IL-1β, WIN 55,212-2 or vehicle were added subsequently and the incubation was continued for 8 h. (B–D) Impact of WIN 55,212-2 on the activation of p38 MAPK (B), p42/44 MAPK (C) and JNK (D) under basal and IL-1β-modulated conditions. Cells were incubated with WIN 55,212-2 at 10 μM for the indicated times in the presence or absence of IL-1β. Percent control (A–D) represents comparison with vehicle-treated cells (100%) in the absence of test substance. Values shown in B–D represent densitometric analyses of blots with the phosphorylated forms being normalized to the respective unphosphorylated form. ß-actin is shown as loading control. All values are means + SEM of n = 3 per group. *P < 0.05, **P < 0.01, ***P < 0.001 vs. corresponding vehicle control; ##P < 0.01, ###P < 0.001 vs. IL-1β, ANOVA plus post hoc Bonferroni test.