Figure 7. Impact of nSMase spiroepoxide inhibitor on IL-1β-induced activation of p38 MAPK (A) and JNK (B).

Cells were pretreated with nSMase spiroepoxide inhibitor (10 μM) for 1.5 h. IL-1β or vehicle were added subsequently and the incubation was continued for 1 h. Percent control represents comparison with vehicle-treated cells (100%) in the absence of test substance. Values represent densitometric analyses and are means + SEM of n = 3 blots per group with the phosphorylated forms being normalized to the respective unphosphorylated form. ß-actin is shown as loading control. ***P < 0.001 vs. corresponding vehicle control; ^##P < 0.01 vs. IL-1β, ANOVA plus post hoc Bonferroni test.