Figure 1. The effect of sal B on cell viability and proliferation in CRC cell lines.

(A) The chemical structures of Sal B. (B) The cell viability of HCT116 cells was measured via the MTT assay after Sal B treatment. The experiments were performed in triplicate. (C) The cell viability of HT29 cells was measured via the MTT assay after Sal B treatment. The experiments were performed in triplicate. (D) Representative cell morphological changes are detected by light microscopy; characteristic morphological features of cell death were observed, including detachment and cell shrinkage. (E) Representative colony formation assay by monolayer culture.