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. 2016 Aug 9;7(38):61562–61574. doi: 10.18632/oncotarget.11146

Figure 5. PSG9 increases the nuclear retention of SMAD4.

Figure 5

A. PSG9 increased the nuclear retention of SMAD4 in stable transfected pcPSG9 HT-29 cells compared with stable transfected pcDNA3.1 cells. B. SMAD4 nuclear expression was determined by IF in HCT-116-pcDNA3.1 and HCT-116-pcPSG9 cells after treatment with 10 ng/ml TGF-β for 4 hours. C. PSG9 increased the nuclear retention of SMAD4 in HCT-116 cells. HCT-116-pcDNA3.1 and HCT-116-pcPSG9 cells were treated with 10 ng/ml TGF-β for 4 hours; cells were washed 3 times to remove TGF-β and were then treated with SB431542 for up 4 hours. The cells were harvested at the indicated times, and both the nuclear and cytoplasmic fractions were collected. SMAD4 nuclear and cytoplasmic ratios were analyzed after treatment with SB431542. Data are expressed as the mean±S.D. values from three replicates. D. Depletion of PSG9 reduced the levels of nuclear SMAD4 compared with shCon in SW-480 cells. SW-480 cells were treated with 10 ng/ml TGF-β for 4 hours. Data are expressed as the mean±S.D. values from 2 experiments.