Skip to main content
. 2016 Aug 9;7(38):61562–61574. doi: 10.18632/oncotarget.11146

Figure 6. PSG9/SMAD4 induces the expression of angiogenesis-related genes.

Figure 6

A. An angiogenesis membrane-based antibody array was used to determine human angiogenesis-related proteins in HCT-116-pcDNA3.1 and HCT-116-pcPSG9 cell lysates. B. An antibody array was used to test the levels of angiogenesis-related proteins in SW-480-shControl (Con) and SW-480-shPSG9 cell lysates. Bottom, semi-quantitative data from dot density relative reference (Ref). C. Quantitative PCR determined the mRNA levels of VEGFA, PDGF-AA, GM-CSF, and IGFBP-3. The data represent the mean±S.D. values of at least four replicates. *P<0.05. D. Chromatin immunoprecipitation (ChIP) revealed that the PSG9/SMAD4 complex was bound to the promoters of IGFBP-3, PDGF-AA, GM-CSF, and VEGFA in HCT-116-pcDNA3.1 and HCT-116-pcPSG9 cells.