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. 2016 Aug 11;7(38):61630–61642. doi: 10.18632/oncotarget.11210

Figure 4. p53 mutants enhance TLR3 induced apoptotic responses.

Figure 4

A. Modulation of TLR3 signaling by p53 mutants induces apoptosis. HCT116 p53−/− cells were transfected with WT and mutant p53 vectors. After 24 h cells were incubated with the TLR3 ligand poly(I:C) (5 μg/mL) and apoptosis was analyzed 24 h later with the Annexin V/PI assay. The results correspond to the Annexin positive cells as a marker of apoptosis. B. DXR treatment enhances poly(I:C) induced apoptosis in a p53 mutant-dependent manner. Twenty-four h post transfection, p53 mutant-expressing cells were co-treated with DXR 0.5 μM and poly(I:C). 24 h later apoptosis was evaluated as described. Presented are means and standard deviations from triplicate determinations from 3 independent experiments. * p<0.001 relative to NT condition, ♦ <0.001 relative to DXR and poly(I:C) treatment alone.