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. 2016 Aug 11;7(38):61630–61642. doi: 10.18632/oncotarget.11210

Figure 6. Functional reactivation of mutant p53 modulates TLR3 induced downstream signaling in RAJI cells.

Figure 6

A-E. Reactivation of mutant p53 in RAJI cells by RITA can increase interferon and cytokine responses to the TLR3 ligand poly(I:C). This can be increased further by DXR and to a lesser extent by 5FU. Twenty four h after drug treatment, RAJI cells were incubated for an additional 3 h with or without poly(I:C) (5 μg/mL). Gene expression was assessed by qPCR. F. Combination of RITA with anticancer drugs enhanced TLR3 mediated apoptosis. Apoptosis was analyzed 24 h after poly(I:C) treatment in the presence or absence of RITA (1μM), DXR (0.5 μM) or 5FU (300 μM) by Annexin-V staining as described in the Material and Methods. Presented are the median and SD of 3 independent experiments. *p<0.001 relative to mock condition. Where indicated, p<0.001 relative to RITA without (⋆) or with (♦) poly(I:C) treatment.