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. 2016 Aug 20;7(38):61741–61754. doi: 10.18632/oncotarget.11437

Figure 3. Knockdown of ANRIL suppressed the proliferation and transforming ability of NPC cells.

Figure 3

(A) Knock-down of ANRIL by different siRNAs resulted in reduced ANRIL mRNA expression in SUNE2 and 6-10B cell lines. (B) The MTT assay measured the viability of SUNE2 and 6-10B cells infected with NC or ANRIL-targeting siRNAs. (C) Brud assay of SUNE2 and 6-10B cells infected with NC or ANRIL-targeting siRNAs. This assay was performed in triplicate (left panel). The percentage of Brud positive cells was shown in right panel. (*P < 0.05, **P < 0.01, ***P < 0.001 by the paired t-test) (D) Colony-formation assay of SUNE2 and 6-10B cells infected with NC or ANRIL-targeting siRNAs. This assay was performed in triplicate (left panel); the quantification of colony formation shown in right panel. (*P < 0.05, **P < 0.01, ***P < 0.001 by the paired t-test) (E) The anchorage-independent growth in soft agar of SUNE2 and 6-10B cells infected with NC or ANRIL-targeting siRNAs. This assay was performed in triplicate (left panel); the quantification shown in right panel. (*P < 0.05, **P < 0.01, ***P < 0.001 by the paired t-test).