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. 2016 Aug 12;7(38):61775–61788. doi: 10.18632/oncotarget.11240

Figure 6. Ewing sarcoma cells lose the repressive H3K27me3 mark at the CXCR4 promoter in response to stress.

Figure 6

A. qRT-PCR shows that serum starvation resulted in upregulated expression of CXCR4 expression in three of four Ewing sarcoma cell lines. B. CHIP-qPCR confirmed diminished H3K27me3 enrichment at the CXCR4 promoter in cells with upregulated transcript expression. C. No change in H3K4me3 enrichment was induced by serum deprivation. D. TC32 cells were FACS-sorted to isolate CXCR4 negative cells which were then placed into 10% FBS or serum deprived conditions for 24 hours. E. CXCR4 mRNA expression was induced by serum deprivation in CXCR4 negative (p<0.005) and also in CXCR4 positive cells (p=0.07) F. Loss of H3K27me3 was evident in CXCR4 negative but not CXCR4 positive cells following serum deprivation. G. Serum withdrawal had no impact on H3K4me3 enrichment at the CXCR4 promoter in either CXCR4 negative or CXCR4 positive cells. Data are represented as mean ± SEM (n=3 for CXCR4 expression, n=2 or 3 for ChIP experiments). *. P <0.05 ** P <0.005 as compared to controls.