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. 2016 Aug 20;7(38):61789–61805. doi: 10.18632/oncotarget.11442

Table 6. PCR Primers.

Identifier Experiment Sequencea
CK2α-LCK-FOR-Gen1-H2 RNAi-CK2 response TCATGAGCACAGAAAGCTACGAcTAATCb
CK2α-LCK-REV-Gen1 RNAi-CK2 response ATACAACCCAAACTCCACATATCC
CK2α-LCK-Internal RNAi-CK2 response TGTCCGAGTTGCTTCCCGATACTTc
CK2αp′-LCK-FOR-Gen1-H2 RNAi-CK2 response GGAGTTTGGGCTGTATGTTAGCaAGCACb
CK2αp′-LCK-REV-Gen1 RNAi-CK2 response GTACCCAGAACCTTGGCAATG
CK2αp′-LCK-Internal RNAi-CK2 response TGGACAGGACAACTATGACCAGCTc
LCK-fw Bioavailability CGAGATACAACCCAAACTCCA
U6-fw Bioavailability CTCGCTTCGGCAGCACA
miRNA-rev Bioavailability GCAGGGTCCGAGGTATTC
SLPoly(A) Bioavailability GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGG ATACGACAAAAAAAAAAAAAAAAAAVNd
SLPoly(A)-LCK Bioavailability GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGA TACGACAAAAAAAAAAAAAAAAAAATGT
a

Upper case letters = DNA; lower case letters = RNA.

b

3′ C3 spacer.

c

Internal reporter = FAM; Quenchers = Internal ZEN & 3′ Iowa Black FQ.

d

V = A, C or G; N = A, C, G or T.