Table 6. PCR Primers.
Identifier | Experiment | Sequencea |
---|---|---|
CK2α-LCK-FOR-Gen1-H2 | RNAi-CK2 response | TCATGAGCACAGAAAGCTACGAcTAATCb |
CK2α-LCK-REV-Gen1 | RNAi-CK2 response | ATACAACCCAAACTCCACATATCC |
CK2α-LCK-Internal | RNAi-CK2 response | TGTCCGAGTTGCTTCCCGATACTTc |
CK2αp′-LCK-FOR-Gen1-H2 | RNAi-CK2 response | GGAGTTTGGGCTGTATGTTAGCaAGCACb |
CK2αp′-LCK-REV-Gen1 | RNAi-CK2 response | GTACCCAGAACCTTGGCAATG |
CK2αp′-LCK-Internal | RNAi-CK2 response | TGGACAGGACAACTATGACCAGCTc |
LCK-fw | Bioavailability | CGAGATACAACCCAAACTCCA |
U6-fw | Bioavailability | CTCGCTTCGGCAGCACA |
miRNA-rev | Bioavailability | GCAGGGTCCGAGGTATTC |
SLPoly(A) | Bioavailability | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGG ATACGACAAAAAAAAAAAAAAAAAAVNd |
SLPoly(A)-LCK | Bioavailability | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGA TACGACAAAAAAAAAAAAAAAAAAATGT |
Upper case letters = DNA; lower case letters = RNA.
3′ C3 spacer.
Internal reporter = FAM; Quenchers = Internal ZEN & 3′ Iowa Black FQ.
V = A, C or G; N = A, C, G or T.