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. 2016 Aug 12;7(38):61806–61819. doi: 10.18632/oncotarget.11241

Figure 4. High basal intracellular ER stress was important for PRIMA-1-induced toxicity.

Figure 4

A. mRNA expression of ER stress markers after U266 was treated with either tunicamycin or PRIMA-1 or combination of both. For combination treatment, U266 was pre-treated with 10ug/mL tunicamycin for 6 hours to induce unfolded protein accumulation. Cells were washed twice with PBS to rid of tunicamycin before fresh PRIMA-1 was added to the cells for another 24 hours. B. Overlayed-histogram of annexin-V-positivity of U266 cells after indicated treatments as analysed on flow cytometer. Analysis was done on Flowjo (Ashland, Oregon, USA). C. Quantification of annexin-V positive cells of U266 after indicated treatments. ** p<0.01