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. 2016 Aug 12;7(38):61806–61819. doi: 10.18632/oncotarget.11241

Figure 6. PRIMA-1-induced-p73 was associated with demethylation of TP73.

Figure 6

A. Methylation specific PCR was performed after the cells were treated with either DMSO, PRIMA-1 (25uM) or 5-Aza (2uM, 24 hours). Methylated and unmethylated p73 promoter specific primers were used for PCR amplification. 5-Aza: 5-Azacytidine (positive control). B. mRNA expression of DNMT1 after PRIMA-1 (25uM) treatment at the indicated time points. C. Protein expression of DNMT1 after PRIMA-1 treatment (25uM) at the indicated time points.