Table 3. Summary of read number, coverage, and mapping results of the two NGS platforms.
Characteristics | Illumina MiSeq |
Ion torrent PGM |
---|---|---|
Flow cell/chip type | Standard | 316 |
Total no. of reads | 14M | 915k and 1.25M |
Mean read length | 99.3bp | 99.24bp |
Average quality of reads (Phred) | 33.79 | 24.24 |
Average no. of reads/sample | 247,749 | 99,774 |
%mapped reads | 76% | 88% |
Coverage, mean [min, max] | 1,262 [171, 3642] | 439 [75, 1326] |
% of target area with ≥ 50 fold coverage | 99.93 | 99.68 |
% of insertions | 0.01 | 1.27 |
% of deletions | 0.04 | 0.67 |
% of mismatches | 0.29 | 0.23 |
All values are average calculations from the 20 analyzed samples.