Table 2. Summary: Immunophenotyping analyses of thymic lymphoma (TL) cells.
| Genotype | Mouse ID | TdT1 | CD32 | CD43 | CD83 | Tcra/d transloc. | Ightransloc. |
|---|---|---|---|---|---|---|---|
| Trp53+/− | 3 | + | + | + | + | N | Y |
| 29 | + | + | − and mod | + | n.d. | n.d. | |
| 32 | + | mod | − and + | − | Y | Y | |
| 44 | + | mod/+ | − and + | + | Y | Y | |
| 44, P154 | n.d. | mod | − | + | n.d. | n.d. | |
| 102 | + | mod | − | + | Y | N | |
| 102, P164 | n.d. | mod | − | + | n.d. | n.d. | |
| 122 | + | + and − | + | + | Y | Y | |
| Ly-1915 | + | + | −/mod | + | n.d. | n.d. | |
| Ly-2005 | + | − and + | − | + | n.d. | n.d. | |
| Ly-2335 | + | + | + and − | + | n.d. | n.d. | |
| Trp53+/− Nucks1+/− | 39 | + | + | mod/+ | + | N | N |
| 79 | + | mod | − | − and mod | N | Y | |
| 79, P154 | n.d. | − | − | − | n.d. | n.d. | |
| 82 | + | + | − and mod | + | N | N | |
| 82, P154 | n.d. | + | − | + | n.d. | n.d. | |
| 121 | + | mod | − | − | n.d. | n.d. | |
| 131 | + | +/mod | − and + | − and + | N | N | |
| 153 | + | +/mod | + | + | Y | N | |
| 162 | + | + | − | − and mod | N | Y | |
| 180 | + | − and + | − | − | N | Y | |
|
Trp53+/− Nucks1−/− |
573 | n.d. | + | − and + | − and + | n.d. | n.d. |
| 573, P174 | n.d. | + | − | − | n.d. | n.d. | |
| 576 | n.d. | + | − and + | − and + | n.d. | n.d. |
1
TdT was examined by IHC only.
2
CD3 immunophenotype was assessed by FACS using isolated primary TL cells, and by IHC using FFPE sections of TLs.
3
CD4 and CD8 were examined by FACS using isolated primary TL cells.
4
Established primary TL cell lines by sub-culturing: cells were split 1:10 ratio to maintain growth at a density ~5 × 10e5 cells/ml and counted as one passage.
5
These cell lines were derived from TLs of Trp53+/− 129S1/SvImJ mice irradiated with 4Gy TBI.
Definitions: “-”: negative; “mod”: positive, but with moderate intensity when compared to “+”; “+”: positive with good intensity; n.d.: not determined.