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. 2016 Aug 22;7(38):62019–62033. doi: 10.18632/oncotarget.11495

Figure 2. FHC-silencing confers a more malignant phenotype through an increase in cellular proliferation ability and glucose uptake.

Figure 2

Cell proliferation and viability, measured by MTT assay, is higher in FHC-silenced compared to non-silenced SKOV3 cells at 24, 48 and 72 h. FHC reconstitution leads to a significant reduction of SKOV3 shFHC cell proliferation at each time point. All the experiments were performed in triplicate (data are represented as mean +/− SD) and the values are given as % of MTT metabolization over the cell type used as control. *p value ≤ 0.05 compared with SKOV3 shScr; °p value ≤ 0.05 compared with SKOV3 shFHC (A). FHC-silenced SKOV3 cells exhibit significant increased glucose uptake than SKOV3 control cells. While at 24 h no differences in glucose concentration in culture media are detectable, at 48 and 72 hours glucose concentration is significantly lower in SKOV3 shFHC compared to SKOV3 shScr cells. *p value ≤ 0.05. When FHC expression is restored, glucose SKOV3 cells exhibit a significant reduced glucose uptake at 48 and 72 hours. °p value ≤ 0.05. The experiment was performed in triplicate and data are represented as mean +/− SD (B).