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. 2016 Aug 22;7(38):62019–62033. doi: 10.18632/oncotarget.11495

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Copyright: © 2016 Lobello et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Wound healing assay was conducted to measure migration capacity of control (SKOV3 shScr), FHC-silenced (SKOV3 shFHC) and FHC-restored (SKOV3 shFHC^pc3FHC) cells. FHC-silencing promotes cellular migration at 4, 6, 12 and 24 h. This ability is reduced by transiently FHC-reconsitution. Wound size was quantified by ImageJ 64 software. Images and quantification are representative of three different experiments and the values are given as mean +/− SD. *p value ≤ 0.05 compared with SKOV3 shScr. °p value ≤ 0.05 compared with shFHC.