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. 2016 Aug 22;7(38):62019–62033. doi: 10.18632/oncotarget.11495

Figure 4. FHC silencing induces EMT in SKOV3 cells.

Figure 4

mRNA (A) and protein (B) expression levels of EMT markers: ZEB1, Vimentin and E-cadherin. ZEB1 is significantly over-expressed in SKOV3 shFHC compared to scrambled SKOV3 control cells at both mRNA and protein levels. The expression of Vimentin and E-cadherin, a mesenchymal and epithelial marker respectively, are inversely correlated. FHC-silencing significantly decreases epithelial marker expression while increasing the mesenchymal one. FHC-reconstitution significantly decreases Vimentin and ZEB-1 mRNA expression levels and increases, albeit without reaching a statistical significance, E-cadherin mRNA expression levels. The experiments were performed in triplicate. qPCR results are shown as mean +/− SD. *p value ≤ 0.05 compared with SKOV3 shScr. °p value ≤ 0.05 compared with shFHC.