Figure 5. Enhanced spheroidogenesis ability of FHC-silenced SKOV3 cells.
SKOV3 shScr and SKOV3 shFHC cells were cultured in low-attachment conditions and formation of spheroids was observed. Photographs of representative spheroids from each cell type (A). Tumour spheroid number, counted at day 6 after each propagation. Results are reported as the average of 4 different fields (B). FHC-silencing induced a significant increase in spheroids number and size at each generation. Results are representative of three different experiments.
