Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Aug 22;7(38):62019–62033. doi: 10.18632/oncotarget.11495

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © 2016 Lobello et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PMC Copyright notice

mRNA expression of stemness markers was evaluated by qRT-PCR. NANOG, SOX2, OCT4 expression increase with statistical significance in SKOV3 silenced cells compared to non-silenced SKOV3 cells. ALDH expression, in particular, is significantly higher in SKOV3 cells after FHC-silencing compared to scrambled SKOV3 control cells. Nanog and ALDH mRNA expression levels are significantly down-regulated after FHC-reconstitution in FHC-silenced cells.*p value ≤ 0.05 compared with SKOV3 shScr. °p value ≤ 0.05 compared with shFHC. qRT-PCR results are shown as means +/− SD (A). Surface stemness markers were evaluated by Flow cytometry analysis. CD34, CD117 and CD133 surface stemness markers are up-regulated in SKOV3 silenced cells compared to non-silenced cells. Only CD34 is significantly up-regulated in SKOV3 shFHC compared to SKOV3 shScr either in adherent or spheroid conditions; CD117 is significantly up-regulated in FHC-silenced spheroids compared to non-silenced spheroids at second and third generations while no statistical significance is observed for CD133 differential expression. The experiment was performed in triplicate and data are shown as mean +/− SD (B).