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. 2016 Aug 22;7(38):62019–62033. doi: 10.18632/oncotarget.11495

Figure 7. FHC intracellular amount affect migration ability of SKOV3 cells via modulation of miR-125b expression.

Figure 7

miR-125b expression level, measured by TaqMan Assay, is significantly reduced after FHC-silencing in SKOV3 cells (A). miR-125b mimic and scrambled control were transfected into SKOV3 shFHC cells. Cells transfected with miR-125b showed a higher level of miR-125b expression compared to scrambled control (B). Images of wound healing assay in shScr, shFHC and shFHCmiR-125b mimic SKOV3 cells. Transient over-expression of miR-125b inhibited the migration of FHC-silenced SKOV3 cells at 12, 17 and 20 h. The wound opening was quantified with ImageJ 64 software (C). miR-146a and miR-150, detected by TaqMan assay, are up-regulated in the second generation of SKOV3 shFHC spheroids compared to controls (D). All data shown are the mean +/− SD of two different experiments. *p value ≤ 0.05.