Figure 3. TNF-α increase MMP-13 expression and activity in lung cancer cells.

Cells were treated with TNF-α (2 ng/ml) a-c. or TNF-α silencing d-e. and MMP-13 expression (a-b, d-e) or activity (c) was determined by western blot (a, d), RT-qPCR (b, e) or ELISA (c), respectively. Data are presented as the mean±SEM, n=3. **p<0.01, ***p<0.001, Student t test. One representative from three experiments is shown. The immunoblots were cropped to improve the clarity and conciseness of the presentation.