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. 2016 Aug 19;7(38):62224–62239. doi: 10.18632/oncotarget.11406

Figure 3. TIPE2 overexpression inhibited the colony formation but not the proliferation of NSCLC cells.

Figure 3

A. and B. After transfection of mock or wild type TIPE2 plasmid, CCK8 assays were conducted with a duration of 5 days. C. and D. After TIPE2 overexpression, TNF-α was used to stimulate the proliferation of tumor cells, then CCK8 assays were conducted at 24 hours, 48 hours and 72 hours after stimulation. E. and F. Two weeks after transient PRK5-TIPE2 plasmid transfection, colonies that consisted of more than 50 cells were counted. Data in (A) to (F) are mean±SD from three independent experiments with similar results. *, P<0.05; **, P<0.01.