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. 2016 Aug 19;7(38):62224–62239. doi: 10.18632/oncotarget.11406

Figure 6. TIPE2 suppressed the colony formation, migration and invasion of NSCLC cells via inhibiting Rac1 pathway.

Figure 6

A. H1975 and A549 cells treated with different concentration of NSC23766, a Rac1 inhibitor, were used for migration assays. B. H1975 and A549 cells co-transfected with Rac1 specific siRNA and mock or TIPE2 plasmids were used for migration assays. C. Total Rac1 expression in H1975 and A549 after transfected with mock and wild type TIPE2 plasmids was detected by western blot. D. After transfected with mock, wild type TIPE2 and mutant TIPE2 plasmids respectively, cell lysates of H1975 and A549 were used for PAK-GST pull down assays. Activated Rac1 that pulled down as well as total Rac1 in the lysates were detected by western blot analysis. E. H1975 and A549 cells that transfected with mock, wild type TIPE2 and mutant TIPE2 plasmids were used for migration and invasion assays. F. H1975 and A549 cells that transfected with mock, wild type TIPE2 and mutant TIPE2 plasmids were used for colony formation assays. Data are mean±SD and the results shown were representative of 3 independent experiments. Five fields of cells in the lower compartment were counted in Transwell assays (200×magnification). *, P<0.05; **, P<0.01; ***, P<0.001.