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. 2016 Aug 19;7(38):62224–62239. doi: 10.18632/oncotarget.11406

Figure 8. TIPE2 decreased F-actin polymerization, VEGF expression and VEGF-A secretion via inhibiting Rac1 pathway.

Figure 8

A. After transfected with mock, wild type TIPE2 and mutant TIPE2 plasmids respectively, F-actin in H1975 cells was stained by TRITC-conjugated phalloidin and observed with laser confocal microscopy. The “% decrease in F-actin”=[(F-actin in untreated cells–F-actin in mock, wild type TIPE2 or mutant plasmids treated cells)/F-actin in untreated cells]×100. B. The expression of VEGF was detected in H1975 cells and A549 cells by western blot after transfected with mock, wild type TIPE2 and mutant TIPE2 plasmids respectively. C. The expression of VEGF-A that secreted into the supernatant of H1975 and A549 cells, which were transfected with mock, wild type TIPE2 and mutant TIPE2 plasmids previously, were detected by VEGF-A ELISA kit. Data are mean±SD and the results shown were representative of 3 independent experiments. *, P<0.05; **, P<0.01; ***, P<0.001.