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. 2016 Aug 19;7(38):62267–62279. doi: 10.18632/oncotarget.11407

Figure 2. The effect of TTF on cell function is similar to that of IR, and the effect of TTF+IR is synergistic.

Figure 2

Cells were exposed to 24 h of TTF, 5 Gy of γ-rays or 5 Gy of γ-rays followed by 24 h of TTF, indicated as the TTF, IR and TTF+IR treatments, respectively. a, b. Immunocytochemistry staining for H2AX phosphorylation (Ser 139, green) in U373 and U87 cells, measured at 1 h and 24 h after treatment with TTF, IR or TTF+IR. c, d. The mean and standard error of the calculated γ-H2AX foci from 3 experiments measured at 1 h and 24 h after treatment with TTF, IR and TTF+IR. The values represent the means of three experiments ± SD; *p < 0.05, **p < 0.001. e, f. Cell lysates immunoblotted with the indicated antibodies after TTF, measured at 24 h after treatment with TTF, IR or TTF+IR. g, h. Neutral comet assay in the U373 and U87 cell lines, measured at 1 h and 24 h after treatment with TTF, IR or TTF+IR. i, j. Quantitative analysis of tail movements, measured at 1 h and 24 h after treatment with TTF, IR or TTF+IR. The values represent the means of three experiments ± SD; *p < 0.05, **p < 0.001.