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. 2016 Aug 19;7(38):62292–62304. doi: 10.18632/oncotarget.11410

Figure 3. Analysis of potential regulators of XPO5 protein expression.

Figure 3

Western blot analyses after the treatment of the melanoma cell lines Mel Im and Hmb2 with the MEK inhibitors PD98059 and U0126 showed a strong downregulation of XPO5 expression. The p-ERK1/2 staining reflects inhibition of ERK phosphorylation by U0126 treatment in both cell lines compared with the control treatment (DMSO). ERK1/2 antibody staining was used to show overall ERK level. ß-Actin was analyzed in the same protein samples on a second blot (due to similar molecular weights) and served as a loading control.