Figure 2. Mass spectrometric analysis of synthetic CXCL12 and [3-NT⁷]CXCL12.

Chemically synthetized human CXCL12 and [3-NT⁷]CXCL12 were deprotected, folded and subsequently purified using RP-HPLC. Fractions were selected based on purity and correct relative molecular mass (M_r). Shown are the averaged mass spectrum of the final pool of synthetic purified and folded CXCL12 (panel A) and [3-NT⁷]CXCL12 (panel B) with the number of charges for the detected ions, ion intensities and corresponding mass to charge ratios (m/z) for the multiply charged ions. The inserts in panels A and B show the deconvoluted mass spectra with the M_r of the uncharged proteins calculated using the Bruker deconvolution software.