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. 2016 Aug 23;7(38):62503–62519. doi: 10.18632/oncotarget.11526

Figure 5. LAT1 overexpression attenuates NQO1 activation induced cell death.

Figure 5

Cells were pretreated with LAT1 plasmid for 24 h and the efficacy was evaluated by western blot A. The NAD+ level B. Cells were then treated with TSA (40 μM, 24 h) or β-lap (5 μM, 2 h withdraw, 12 h). The mRNA level and enzyme activity of SIRT1 C. The mRNA level of FOXO1 D. The protein levels of Ac-FOXO1 and SIRT1 E. Cytotoxicity F. and apoptosis test G. were performed after cells treated with TSA (40 μM, 48 h) or β-lap (5 μM, 2 h withdraw, 24 h). Data are shown as mean ± SEM of three independent experiments (*P<0.05, **P<0.01, TSA or β-lap treatment compared with control cells; #P<0.05, ##P<0.01, LAT1 plasmid treatment compared with vector treatment).