Figure 1. Regulation of EMT markers and SOCS1 expression by H₂O₂ and effects of NAC on H₂O₂-induced ROS and EMT markers in colon cancer cell lines.

HCT116 p53 +/+ cells were treated with H₂O₂ at 200 μM for different durations A., and for 2 h at increasing doses B., which were then subjected to the immunoblot analysis for SOCS1 and EMT markers. HCT116 p53+/+, HCT116 p53−/−, and HT29(p53mt) colon cancer cell lines were treated with H₂O₂ (200 μM, 2 h) with or without pretreatment of NAC for 1 h at 10 mM, after which EMT marker expressions were analyzed C. The ROS levels in these cells were determined at 30 min post H₂O₂ treatment D.