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. 2016 Aug 23;7(38):62559–62571. doi: 10.18632/oncotarget.11537

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Copyright: © 2016 Jung et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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HCT116 p53 +/+ cells A. and HCT116 p53 +/+ HA & HA-SOCS1 cells B. were treated with H₂O₂ for different durations and antioxidant proteins were analyzed by Western blot. HA & HA-SOCS1 cells were each transfected with shRNA or shTrx and then treated by H₂O₂, after which changes in EMT markers and kinases were analyzed by Western blot at 2 h C. Analysis of intracellular ROS levels was performed for cells in Panel (C) at 30 min after H₂O₂ treatment D.