Figure 6. Role of Src in the H₂O₂-induced intracellular ROS and thioredoxin/Nrf-2 regulation by SOCS1.

HCT116 p53 +/+ cells were transfected with sh or shSrc construct as described in the text and cells were analyzed for ROS levels upon treatment with H₂O₂ at 30 min with or without NAC pretreament A. sh and shSOCS1 cells transfected with sh or shSrc were subject to treatment with H₂O₂. The intracellular ROS levels were determined at 30 min B. Cells in panel B were treated with H₂O₂ for 2 h, harvested to obtain subcellular fractions, and analyzed for the expression of thioredoxin (Trx1), Nrf-2, and EMT markers by immunoblotting C.