Table 1. Phosphorylated amino acid residues identified by mass spectrometry.

In vitro phosphorylated HNF4α was subjected to mass spectrometry analysis. The fragments containing the phosphorylated amino acid residues correspond to phosphorylation sites on the HNF4α protein. Detected phosphorylation sites (serine/threonine residues) in the cryptic fragments are marked bold and underlined. Affected phosphorylation sites appear in the DNA binding domain (DBD), the hinge, the ligand-binding domain (LBD) and the C-terminus of HNF4α, as well.

Sequence	Identified phosphorylation sites	Part of HNF4α
KNHMYSCR	S95	DBD
QNERDRISTRRSSYED	S138/T139	hinge
QNERDRISTRRSSYED	S142/S143	hinge
STRRSSYEDSSLPSINALLQ	S147/S148	hinge
STRRSSYEDSSLPSIN	S151	hinge
EVLSRQITSPVSGIN	T166/S167	LBD
HCPELAEMSRVSIR	S262, S265	LBD
GKIKRLRSQVQVSLED	S313	C-terminus
SAIPQPTITKQE	S451, T457, T549	C-terminus