Fig 10. Artemisinin-mediated knockdown of ERα expression and its consequent effects on Bcl-2 and cyclin D1 expression in MCF-7CC-7 and MCF-7CC-12 cells.
(A) MCF-7CC-7 and MCF-7CC-12 cells were treated with either DMSO or 300 μM artemisinin. Whole cell extracts of these cells were then monitored for Bcl-2, cyclin D1, and γ tubulin protein expression using immunoblotting approaches with epitope-specific antibodies. γ tubulin was used as loading control. Blots are representative of 3 independent trials. (B) Fold changes in ERα levels induced by artemisinin in MCF-7CC cells at selection doses 7 and 12. (C) Fold changes in Bcl-2 levels induced by artemisinin in MCF-7CC cells at selection doses 7 and 12. (D) Fold changes in cyclin D1 levels induced by artemisinin in MCF-7CC cells at selection doses 7 and 12. All values depicted are the mean ± S.E.M. for 3 independent experiments. The significance of artemisinin-induced changes in the expression of the above proteins was assessed using an ANOVA test, followed by a Bonferoni correction. n = 3, *** p< 0.001