REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins
4-Azidophenacyl bromide	Sigma Aldrich	A6057
7-Diethylamino-3-[N-(2-maleimidoethyl)carbamoyl]coumarin (MDCC)	Sigma Aldrich	05019
Purine Nucleoside Phosphorylase	Sigma Aldrich	N8264
7-Methylguanosine	Sigma Aldrich	M0627
0.65mM Phosphorus Standard Solution	Sigma Aldrich	P3869
Adenosine 5′-(β,γ-imido)triphosphate lithium salt hydrate (AMPPNP)	Sigma Aldrich	A2647
Adenosine 5′-diphosphate sodium salt (ADP)	Sigma Aldrich	A2754
Phenol:Chloroform 5:1	Sigma Aldrich	P1944
Sodium Fluoride	Fluka	71519
Beryllium Chloride	Sigma Aldrich (discontinued)	201197
Streptavidin	Pierce	21125
3.05 mm copper grids, 400 lines/inch square mesh	Electron Microscopy Sciences	G400-Cu
Uranyl formate	SPI supplies	16984-59-1
Phusion DNA Polymerase	NEB	M0530
Pyruvate Kinase from rabbit muscle	Roche	10 128 155 001
L-Lactate Dehydrogenase from rabbit muscle	Roche	10 127 230 001
Phospho(enol)pyruvic acid trisodium salt hydrate	Sigma Aldrich	P7002
β-Nicotinamide adenine dinucleotide, reduced disodium salt hydrate	Sigma Aldrich	N8129
Critical Commercial Assays
Thermo Sequenase Dye Primer Manual Cycle Sequencing Kit	Affymetrix	79260
Recombinant DNA
Cysteine-free ScChd1118–1274	This study	N/A
ScChd1118–1274-A1117C	This study	N/A
ScChd1118–1274-H1252C	This study	N/A
ScChd1118–1274-A1250C	This study	N/A
ScChd1118–1274-E493C	This study	N/A
ScChd1118–1274-N459C	This study	N/A
ScChd1118–1274-N650C	This study	N/A
ScChd1118–1274-V721C	This study	N/A
ScChd1118–1274-G743C	This study	N/A
ScChd1118–1274-G238C	This study	N/A
ScChd1118–1274-R237C	This study	N/A
ScChd1118–1274-L1119C	This study	N/A
ScChd1118–1274-N1129C	This study	N/A
ScChd1118–1274-T491C	This study	N/A
ScChd1118–1274-S523C	This study	N/A
ScChd1118–1274-S524C	This study	N/A
ScChd1118–1274-G643C	This study	N/A
ScChd1118–1274-F646C	This study	N/A
ScChd1118–1274-S770C	This study	N/A
ScChd1118–1274	McKnight et al., 2011	N/A
ScChd1118–1014	McKnight et al., 2011	N/A
ScChd11006–1274	Sharma et al., 2011	N/A
XlHistone H2A	Karolin Luger Lab	N/A
XlHistone H2B	Karolin Luger Lab	N/A
XlHistone H3	Karolin Luger Lab	N/A
XlHIistone H4	Karolin Luger Lab	N/A
XlHistone H4-A15C	Geeta Narlikar Lab	N/A
XlHistone H3-C110A	Blaine Bartholomew Lab	N/A
E.coli Phosphate Binding Protein-A197C	Martin Webb Lab	N/A
pGEM-601 (see Table S3)	Jonathan Widom Lab
pGEM-601(LacO-11L) (see Table S3)	Nodelman et al., 2016	N/A
pGEM-601(LacO-11R) (see Table S3)	Nodelman et al., 2016	N/A
pGEM-601(LacO-6R) (see Table S3)	Nodelman et al., 2016	N/A
pJ201-601array-208bpx34 (33N26, 2N61) (see Table S4)	Patel et al., 2011	N/A
pJ201-601array-169bpx16 (0N0) (see Table S4)	This study	N/A
pJ201-601array-178bpx12 (0N33) (see Table S4)	This study	N/A
Sequence-Based Reagents
Primers for nucleosomal DNA constructs are given in Table S2; Templates for nucleosomal DNA constructs are given in Table S3.
80N0 DNA (see Tables S2, S3)	This study	N/A
0N80 DNA (see Tables S2, S3)	This study	N/A
0N63 DNA (see Tables S2, S3)	This study	N/A
11N70 DNA (see Tables S2, S3)	This study	N/A
29N19 DNA (see Tables S2, S3)	This study	N/A
40N40 DNA (see Tables S2, S3)	This study	N/A
Software and Algorithms
ImageJ 1.47v	NIH	https://imagej.nih.gov/ij/
PyMol, (version 1.7)	The PyMol Molecular Graphics System, Schrodinger, LLC	www.pymol.org
Mathematica (version 10)	Wolfram Research	https://www.wolfram.com/mathematica/
EPU software package	FEI
CTFFIND4	Rohou and Grigorieff, 2015	http://grigoriefflab.janelia.org/ctffind4
DOGPICKER	Voss et al., 2009	http://emg.nysbc.org/redmine/projects/software/wiki/DoGpicker
SPIDER	Frank et al., 1996	http://spider.wadsworth.org/spider_doc/spider/docs/spider.html
XMIPP 2.4	Scheres et al, 2008	http://xmipp.cnb.csic.es/twiki/bin/view/Xmipp/WebHome
RELION 1.4	Scheres, 2012; Scheres, 2016	http://www2.mrc-lmb.cam.ac.uk/relion/index.php/Main_Page
Other
HisPrep FF 16/10 (Nickel affinity)	GE	28-9365-51
HisTrap HP, 5ml (Nickel affinity)	GE	17-5248-01
HiTrap SP FF, 5ml	GE	17-5157-01
HiTrap Q FF, 5ml	GE	17-5156-01
HiLoad 16/600 Superdex 200, prep grade	GE	28-9893-35
HiPrep 26/10 Desalting	GE	17-5087-01
HiPrep 16/10 Q FF	GE	17-5190-01
HiPrep 16/10 SP FF	GE	17-5192-01