KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Goat polyclonal anti-mCherry	SICGEN	Cat# AB0040-200; RRID:AB_2333092
Goat polyclonal anti-GFP	SICGEN	Cat# AB0020-200; RRID:AB_2333099
Chicken polyclonal anti-GFP	Aves Labs	Cat# GFP-1020; RRID:AB_10000240
Rabbit polyclonal anti-GFAP	Dako	Cat# Z0334; RRID:AB_10013382
Rabbit polyclonal anti-GFP (for immuno-EM)	Frontier Institute	Cat# GFP-Rb-Af2020; RRID:AB_2571573
Donkey anti-Goat IgG (H+L), Alexa Fluor 488	Thermo Fisher Scientific	Cat# A-11055; RRID:AB_2534102
Donkey anti-Goat IgG (H+L), Alexa Fluor 546	Thermo Fisher Scientific	Cat# A11056; RRID:AB_10584485
Donkey anti-Rabbit IgG (H+L), DyLight 650	Thermo Fisher Scientific	Cat# SA5-10049; RRID:AB_2556629
Donkey anti-Chicken IgY (IgG) (H+L), Cy2	Jackson ImmunoResearch Labs	Cat# 703-225-155; RRID:AB_2340370
Goat anti-Rabbit Fab′ fragment IgG, NANOGOLD (for immuno-EM)	Thermo Fisher Scientific	Cat# N24916; RRID:AB_10104359
Experimental Models: Mouse lines
Tg(Slc1a3-cre/ERT)1Nat/J	Dr. Jeremy Nathans, Johns Hopkins	RRID:IMSR_JAX:012586
B6.Cg-Tg(GFAP-cre/ERT2)505Fmv/J	Jackson Laboratory	RRID:IMSR_JAX:012849
Itpr2tm1Chen/Itpr2+	Dr. Ju Chen, UC San Diego	RRID:MGI:3713675
B6SJL-Tg(SOD1*G93A)1Gur/J	Jackson Laboratory	RRID:IMSR_JAX:002726
B6;129-Gt(ROSA)26Sor(CAG-mGCaMP3)	This paper	N/A
B6;129-Gt(ROSA)26Sortm4(CAG-GFP*)Nat/J	This paper	RRID:IMSR_JAX:021429
B6;129S6-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J	Jackson Laboratory	RRID:IMSR_JAX:007908
Chemicals (Pharmacological compounds)
(S)-3,5-Dihydroxyphenylglycine, DHPG	Tocris	Cat# 0805; CAS:162870-29-3
Adenosine 5′-triphosphate magnesium salt	Sigma-Aldrich	Cat# A9187; CAS:74804-12-9
Bafilomycin A1	Enzo	Cat# BML-CM110-0100; CAS: 88899-55-2
Benzamil	Tocris	Cat# 3380; CAS:161804-20-2
Cadmium chloride	Sigma-Aldrich	Cat# 202908; CAS:10108-64-2
Carbonyl cyanide 4 (trifluoromethoxy)phenylhydrazone (FCCP)	Tocris	Cat# 0453; CAS:370-86-5
Carboxyatractyloside potassium salt	Calbiochem	Cat# 216201; CAS:35988-42-2
CGP 37157	Abcam	Cat# ab120012; CAS:75450-34-9
Cyclosporin A	Tocris	Cat# 1101, CAS:59865-13-3
Dantrolene, sodium salt	Tocris	Cat# 0507; CAS:14663-23-1
DL-TBOA	Tocris	Cat# 1223; CAS:205309-81-5
GSK-7975A	GlaxoSmithKline	N/A; CAS:1253186-56-9
HC 030031	Tocris	Cat# 2896; CAS:349085-38-7
KB-R7943 mesylate	Tocris	Cat# 1244; CAS:182004-65-5
L-(−)-Norepinephrine (+)-bitartrate salt monohydrate	Sigma-Aldrich	Cat# A9512; CAS:108341-18-0
Picrotoxin	Sigma-Aldrich	Cat# P1675; CAS:124-87-8
Rotenone	Tocris	Cat# 3616; CAS:83-79-4
Tetrodotoxin citrate	Abcam	Cat# ab120055; CAS:18660-81-6
Tamoxifen	Sigma-Aldrich	Cat# T5648; CAS:10540-29-1
Thapsigargin	Sigma-Aldrich	Cat# T9033; CAS:67526-95-8
Veratridine	Tocris	Cat# 2918; CAS:71-62-5
Critical Commercial Assays
Neural Tissue Dissociation Kit (P)	Miltenyi Biotec	Cat# 130-092-628
Recombinant DNA
AAV8-hGFAP-mCherry-2A-Δ9UCP1-WPRE	This paper	N/A
Sequence-Based Reagents
Primer: tcaatgggcgggggtcgtt (CMV-E-as)	(Paukert et al., 2014)	N/A
Primer: ctctgctgcctcctggcttct (ROSA26-s)	(Paukert et al., 2014)	N/A
Primer: cgaggcggatcacaagcaata (ROSA26-as)	(Paukert et al., 2014)	N/A
Primer: cacgtgatgacaaaccttgg (CaM-s)	This paper	N/A
Primer: ggcattaaagcagcgtatcc (WPRE-as)	This paper	N/A
Software and Algorithms
ZEN Blue/Black	Zeiss	RRID:SCR_013672
ImageJ	https://imagej.nih.gov/ij/	RRID:SCR_003070
Fiji	http://fiji.sc	RRID:SCR_002285
TurboReg	http://bigwww.epfl.ch/thevenaz/turboreg/	RRID:SCR_014308
MATLAB (R2014b)	MathWorks	RRID:SCR_001622
LIBSVM	http://www.csie.ntu.edu.tw/~cjlin/libsvm/	RRID:SCR_010243
pClamp 9.2	Molecular Devices	RRID:SCR_011323
MiniAnalysis	Synaptosoft Inc.	RRID:SCR_014441
Origin 8.0	OriginLab Corp.	RRID:SCR_014212
Prism 5.0	GraphPad Software	RRID:SCR_002798
Adobe Illustrator CS6	Adobe	RRID:SCR_014198
CaSCaDe	This paper	(see Data S1)