Fig. 2.

Glioma-induced increase in network excitability.

(a) Representative fEPSPs in response to a threshold stimulation intensity and increasing stimulation widths from 20 μs–240 μs in the glioma-injected ipsilateral and contralateral cortices. The slope of the fEPSP was calculated over an area indicated with a grey bar on the rising phase of the fEPSP corresponding to the source (upward slope). (b) The percent maximum fEPSP slope is plotted against the stimulation width to generate input-output curves from the ipsilateral (grey dots) and contralateral (black dots) cortices. The input-output curves were fit with a Boltzmann function which demonstrates a leftward shift in the input-output curve from the ipsilateral cortex (grey line) compared to the contralateral cortex (black line). inset, The half maximum stimulation width (W₅₀), calculated from the Boltzmann fits, was decreased in the ipsilateral cortex (grey bars) compared to the contralateral cortex (black bars). n = 10 slices, 3–4 mice per experimental group. *Denotes a p value <0.05 determined using a Student’s t-test.