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. Author manuscript; available in PMC: 2017 Dec 12.
Published in final edited form as: Angew Chem Int Ed Engl. 2016 Nov 17;55(50):15672–15679. doi: 10.1002/anie.201605429

Figure 4.

Figure 4

Binding activity and selectivity of the peptides in vitro. (a, c, e) Binding curves of δPKC and εPKC, at ~ 75 μg/mL (~1 μM), to the ψMARCKS-Cargo (a), ψDrp1-Cargo (c), and ψIRS1-Cargo (e) peptides. Each peptide selectivity binds to δPKC and not to another novel PKC, εPKC. (b, d, f) Binding assay of increasing amounts of δPKC to ψMARCKS-Cargo (b), ψDrp1-Cargo (d), and ψIRS1-Cargo (f) peptides. Each peptide selectivity binds to δPKC (ψMARCKS-Cargo Kd 8.2 ± 2.3 nM; ψDRP1-Cargo Kd 1.4 ± 0.3 nM; ψIRS1-Cargo Kd 10.2 ± 3.0 nM). The results are from three independent experiments (Table S2). (g–i) Binding assay of increasing amounts of δPKC to ψMARCKS (g), ψDrp1 (h), and ψIRS1 (i), peptides. Each peptide selectivity binds to δPKC (ψMARCKS Kd 3.3 ± 0.9 nM; ψDRP1 Kd 2.9 ± 1.1 nM; ψIRS1 Kd 2.9 ± 1.1 nM).