Figure 1.

A. PCR genotyping of DNA samples from JTMT founder mice. Primers corresponding to the human MTII gene resulted in a 536 bp DNA band, and control primers designed for a region of the FVB background DNA resulted in a 200 bp band. DNA containing the transgene displayed two DNA bands, and the corresponding animals were given the nomenclature JTMT‐1–5. B. Glomerular MT protein expression. Glomeruli were isolated from both wild type FVB and JTMT transgenic mice. The MT protein expression was detected by Western blot as previously reported (Wang et al., 2006).