Skip to main content
. Author manuscript; available in PMC: 2017 May 3.
Published in final edited form as: Pediatr Res. 2016 Nov 3;81(2):376–383. doi: 10.1038/pr.2016.218

Figure 5. CAV1 knockdown increases ECM expression in fASM cells.

Figure 5

fASM cells were transfected with CAV1 or scrambled siRNA prior to exposure to 21% or 50% oxygen. Knockdown of CAV1 increased collagen III expression in 21% oxygen. Both collagen I and III were significantly increased following CAV1 transfection and 50% oxygen exposure. Transfection with scrambled siRNA did not result in significant ECM change from control (data not shown). Protein expression in concentrated media was normalized to cellular lysate protein concentration. Values are presented as mean +/- SEM. Legend: Black=21% oxygen, Light Gray= 21% oxygen transfected with CAV1 siRNA, Dark Gray= 50% oxygen, Cream= 50% oxygen transfected with CAV1 siRNA. * indicates significant difference from 21% control.