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. Author manuscript; available in PMC: 2017 May 3.
Published in final edited form as: Pediatr Res. 2016 Nov 3;81(2):376–383. doi: 10.1038/pr.2016.218

Figure 7. Impact of CAV1 knockdown on MMP activity and MMP and TIMP expression.

Figure 7

fASM cells were exposed to 21% with or without CAV1 siRNA transfection. A) Conditioned media was collected, concentrated, and analyzed via gelatinase zymograph for MMP2 and MMP9 activity. There was no effect of CAV1 siRNA transfection on MMP activity. B) Conditioned media was collected, concentrated and analyzed via western blot for MMP and TIMP expression. Protein expression in the conditioned media was normalized to the protein concentration of the corresponding cellular lysate. There was no effect of CAV1 siRNA transfection on MMP or TIMP protein expression in concentrated media. Values are means ± SEM from n of 4 samples. * indicates significant difference from 21% O2 control with p<0.05.