Fig. 3.
Effect of increasing platelet stiffness on shear-mediated platelet activation. Platelets stiffened via enhancing platelet actin polymerization and organization, via exposure to FTY720 S-phosphonate (Tysiponate, TYS, 1 µM, 10 min.) demonstrate increased responsiveness to 70 dyne/cm2 (70t) shear vs. non-TYS control (left). Platelets made less stiff, via inhibition of actinmyosin filaments and organization, after exposure to permanent inhibitor of myosin light chain kinase (PIK, 250 µM, 10 min.), demonstrate reduced responsiveness to shear vs. non-PIK control (right).