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. 2017 Feb 15;8:159. doi: 10.3389/fimmu.2017.00159

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Copyright © 2017 Peng, Zhu, Zhang, Shi, Feng, Liu, Huang and Zheng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Smo was highly expressed in cultured fibroblast-like synoviocytes (FLSs) from rheumatoid arthritis (RA) patients. RA-FLSs, OA-FLSs, and FLSs from patients with knee traumatic injury were isolated and cultured. The expression of Smo mRNA was detected by real-time PCR. Relative quantification of gene expression was performed by the 2^−ΔΔCt method (A). The expression of Smo protein was examined by Western Blot analysis (B). The results represent the mean ± SD of three independent experiments. *P < 0.05 versus control group.